Double Labeling with Fluorescence In Situ Hybridization in Drosophila Whole-Mount Embryos

Double labeling with fluorescence in situ hybridization in Drosophila whole-mount embryos.

Previously, we described a technique for fluorescence in situ hybridization (FISH) in whole-mount Drosophila embryos using fluoresceinlabeled RNA probes (2). This technique provides several advantages over conventional alkaline phosphatasebased detection methods. For example, a fluorescent label is nondiffusible and so allows higher-resolution imaging. Furthermore, it allows the use of confocal...

Double labeling for whole-mount in situ hybridization in mouse.

1.Brugal, G. 1995. Quantitative microscopy and tumor cell proliferation. Bull. Cancer 511S517S. 2.Doyle, A., J.B. Griffiths and D.G. Newell. 1993. Cell and Tissue Culture: Laboratory Procedures. John Wiley & Sons International, Chichester, UK. 3.Gerdes, J., U. Schwab, H. Lemke and H. Stein. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cel...

Combined fluorescence in situ hybridization and immunocytochemistry double labeling in whole-mount Manduca sexta tissue.

Whole-mount fluorescence in situ hybridization and antibody staining of Drosophila embryos.

Whole-mount RNA in situ hybridizations with digoxigenin-conjugated probes and alkaline phosphatase biochemistry have been used widely for many years to map expression pattern domains in the Drosophila embryo. To capitalize on the number of molecular markers in the central nervous system (CNS) and to enable expression analysis at the single-cell level, fluorescence in situ hybridization procedur...

Whole-mount in situ hybridization to mouse embryos.

The mouse is well-established as the major animal model for the study of mammalian development. Rapid progress in large-scale cDNA and also genomic sequencing projects is identifying new mouse genes at an unprecedented rate. As a first step toward understanding the function of these novel genes, it is important to determine their developmental expression pattern. Here we provide a reliable, sen...